Beta-Amyloid 1-40, HFIP
 3XFlag peptide
 MOG 35-55
 Beta-Amyloid 1-42, HFIP
 Beta-Amyloid 1-42, TFA
 Beta-Amyloid 1-40, TFA

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Beta-Amyloid 1-42 | Aβ1–42, Human

Beta-Amyloid 1-42 | Aβ1–42, Human




 
    货号 数量 价格(元) 货期
    A-42-T-1 1 mg ¥800.00 1~2天
    A-42-T-5 5 mg 询价 1~2天
    A-42-T-10 10 mg 询价 1~2天

  英文别名: Aβ42 | Aβ1-42 | Abeta42 | Abeta 1-42 | Amyloid-β42 | β-Amyloid 1-42
  中文名: β-淀粉样多肽 1-42 | Beta-淀粉样多肽 1-42 | Beta-淀粉样多肽 -42 | Β-淀粉样多肽(1-42) | Beta-Amyloid多肽(1-42)
  纯度: >95% by HPLC
  序列(单字母): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
  (三字母序列): Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile- Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala
  CAS No.: 107761-42-2
  分子式: C203H311N55O60S
  分子量: 4514.14
  外观: 白色粉末
  等电点: 5.2
  净电荷(PH=7): -2.7
  平均亲水性: -0.1
  亲水残基比例: 31%
  盐: TFA
  来源: 化学合成
  储存条件(冻干粉):

建议冻干粉在-20℃或-80℃冷冻干燥保存。

由于多肽的每次冻融均会引起部分失活,如果需要液体保存,须分装后-20℃或-80℃冻存,以避免反复冻融,建议2-4周内用完。

  溶解条件: Acetic acid/water (3:2) or DMSO
  运输条件: 常温(加冰袋)
  描述: Alzheimer's disease is a common neurodegenerative, progressive, and fatal disorder. Generation and deposition of amyloid beta (Aβ) peptides associate with its pathogenesis and small soluble Aβ oligomers show the most pronounced neurotoxic effects and correlate with disease initiation and progression.
Beta amyloid 1-42 is a 42-amino acid peptide which plays a key role in the pathogenesis of Alzheimer, even slightly increased amounts of this Aβ(1–42) might be sufficient to cause Alzheimer's disease (AD).







Beta amyloid proteins 聚集指南(以下方案仅供参考,请根据您的具体实验需求进行调整):








1. 将Aβ肽(1-42)粉末溶于冷的六氟-2-丙醇(HFIP)。肽溶液在室温下孵化至少1小时,以建立单体化和随机化的结构。(Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.)


2. 蒸发掉HFIP,将得到的多肽以薄膜形式保存在-20或-80℃。(The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.)


3. 将得到的薄膜以5 mM的浓度溶解于无水DMSO中,然后用涡旋方式稀释到适当的浓度和缓冲液(不含血清和酚红的培养基)中。(The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.)


4. 接下来,该溶液在4-8℃下老化48h。然后在4-8 ℃下以14000g离心10分钟;可溶性低聚物在上清液中。将上清液稀释10-200倍用于后续实验。(Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.)


以上方法因后续不同应用而异(Methods vary depends on the downstream applications.)。


注意:老化后由于在溶液中聚集形态不稳定,建议现配现用。









储备液配制: 














不同实验动物依据体表面积的等效剂量转换表(以下信息来源于公开文献,仅供参考):




 
 
   Product Citations Einfluss der sauren Sphingomyelinase auf die Makrophagenaktivierung durch Alzheimer Amyloid Protein (2012)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Herrn Prof. Dr. Fa?bender, Herrn Dr. Liu und Herrn Dipl.-Biol. Xie danke ich vielmals
Journal: der Medizinischen Fakult?t der UNIVERSIT?T DES SAARLANDES
 


Transgenic Overexpression of the Disordered Prion Protein N1 Fragment in Mice Does Not Protect Against Neurodegenerative Diseases Due to Impaired ER Translocation (2020)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Behnam Mohammadi, Luise Linsenmeier,Mohsin Shafiq, etc.
Journal: Journal of Molecular Neurobiology
Doi: https://doi.org/10.1007/s12035-020-01917-2
     
    An analog derived from phenylpropanoids ameliorates Alzheimer's disease–like pathology and protects mitochondrial function (2019)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Shichao Huang,Xin Caob, Yue Zhou, Fuchun Shi, Gang Pei, etc.
Journal: Neurobiology of Aging
Doi: https://doi.org/10.1016/j.neurobiolaging.2019.05.002
     
    Modulation of OSCP mitigates mitochondrial and synaptic deficits in a mouse model of Alzheimer’s pathology(2019)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Esha Gauba, Shaomei Sui, Jing Tian, Christopher Driskill, etc.
     
    The Neuroprotective Action of Amidated-Kyotorphin on Amyloid β Peptide-Induced Alzheimer’s Disease Pathophysiology(2020)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List:  Rita F. Belo, Margarida L. F. Martins, Liana Shvachiy, etc.
Journal: Front Pharmacol. 2020; 11: 985.
Doi: 10.3389/fphar.2020.00985
     
    A pyrene-inhibitor fluorescent probe with large Stokes shift for the staining of Aβ1–42, α-synuclein, and amylin amyloid fibrils as well as amyloid- containing Staphylococcus aureus biofilms(2019)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Alejandro Mahía, María Conde-Giménez, Sandra Salillas, etc.
Journal: Analytical and Bioanalytical Chemistry
Doi: https://doi.org/10.1007/s00216-018-1433-8
     
    The Effects of Cardiotrophin-1 on Early Synaptic Mitochondrial Dysfunction and Synaptic Pathology in APPswe/PS1dE9 Mice(2017)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Wang, Dongmei; Liu, Xiaozhuan; Liu, Yumei; Li, Sanqiang; Wang, Chenying
Journal: Journal of Alzheimer's Disease, vol. 59, no. 4, pp. 1255-1267, 2017
DOI: 10.3233/JAD-170100
     
    Effect of Cholesterol on Membrane Fluidity and Association of Aβ Oligomers and Subsequent Neuronal Damage: A Double-Edged Sword(2018)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Eduardo J. Fernández-Pérez, Fernando J. Sepúlveda, etc.
Journal: Front. Aging Neurosci., 03 August 2018
Doi: https://doi.org/10.3389/fnagi.2018.00226
     
    A Fluorescence‐Based Sensor Assay that Monitors General Protein Aggregation in Human Cells(2018)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Marisa Pereira, Diogo Tomé, Ana S. Domingues, Ana S. Varanda, etc.
Journal: Journal of Biotechnology
Doi: https://doi.org/10.1002/biot.201700676
     
    Amyloid aggregation and membrane activity of the antimicrobial peptide uperin 3.5(2018)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Lisandra L. Martin, Clemens Kubeil, Stefania Piantavigna, Tarun Tikkoo, etc.
Journal: Journal of peptide science
Doi: https://doi.org/10.1002/pep2.24052
     
    Rapamycin protects against Aβ-induced synaptotoxicity by increasing presynaptic activity in hippocampal neurons(2014)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: A.E.Ramírez, C.R.Pacheco,L.G.Aguayo
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
Doi: https://doi.org/10.1016/j.bbadis.2014.04.019
     
    Coat protein I depletion-associated Golgi fragmentation in an Alzheimer's disease model
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Mi Jeong Kim, A Reum Je, Hyo-Jeong Kim, etc.
Journal: Journal of Animal Cells and Systems
Doi: https://doi.org/10.1080/19768354.2014.984756
     
    Alzheimer's Aβ interacts with cellular prion protein inducing neuronal membrane damage and synaptotoxicity
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Christian Peters, María Paz Espinoza, Scarlet Gallegos, etc.
Journal: Neurobiology of Aging
Doi: https://doi.org/10.1016/j.neurobiolaging.2014.11.019
     
    Levetiracetam inhibits oligomeric Aβ-induced glutamate release from human astrocytes(2016)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Sara Sanz-Blasco, Juan C. Pi?a-Crespo, Xiaofei Zhang, Scott R. McKercher, etc.
Journal: Journal of Neuroreport
Doi: 10.1097/WNR.0000000000000601
     
    Multifunctional Curcumin-Nanocarriers Based on Host-Guest Interactions for Alzheimer Disease Diagnostic(2015)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Lamesh Ramdani, Romain Bourboulou, Mounir Belkouch, etc.
Journal: Journal of Nanomedicine & Nanotechnology
Doi: 10.4172/2157-7439.1000270
     
    The membrane-active amphibian peptide caerin 1.8 inhibits fibril formation of amyloid β1-42(2015)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Yanqin Liu, Tianfang Wang, Antonio N.Calabrese, John A.Carver, etc.
Journal: Journal of Peptides; Volume 73, November 2015, Pages 1-6
Doi: https://doi.org/10.1016/j.peptides.2015.08.004
     
    Effects of dipole modifiers on channel-forming activity of amyloid and amyloid-like peptides in lipid bilayers(2015)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: S. S. Efimova, V. V. Zakharov & O. S. Ostroumova.
Journal: Cell and Tissue Biology volume 9, pages250–259(2015)
Doi: https://doi.org/10.1134/S1990519X15030049
     
    Amyloid-beta oligomerization is associated with the generation of a typical peptide fragment fingerprint(2016)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Nikita Rudinskiy | Christophe Fuerer | Davide Demurtas | Sebastian Zamorano, etc.
Journal: Alzheimer's & Dementia; Volume 12, Issue 9, September 2016, Pages 996-1013
Doi: https://doi.org/10.1016/j.jalz.2016.03.011
     
    Differential Membrane Toxicity of Amyloid-β Fragments by Pore Forming Mechanisms(2016)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: Peters, Christian | Bascu?án, Denisse | Opazo, Carlos | Aguayo, Luis G.
Journal: Journal of Alzheimer's Disease, vol. 51, no. 3, pp. 689-699, 2016
     
    Crystallization and preliminary X-ray diffraction analysis of the Fab portion of the Alzheimer's disease immunotherapy candidate bapineuzumab complexed with amyloid-[beta](2014)
Product Name: Beta-amyloid 1-42(GenicBio)
Author List: G. A. N. Crespi, D. B. Ascher, M. W. Parker and L. A. Miles
Journal: CRYSTALLIZATION COMMUNICATIONS
     
    Exosomal cellular prion protein drives fibrillization of amyloid beta and counteracts amyloid beta‐mediated neurotoxicity(2015)
P
roduct Name: Beta-amyloid 1-42(GenicBio)
Author List: Clemens Falker Alexander Hartmann Inga Guett Frank Dohler Hermann Altmeppen, etc.
Journal: Journal of Neurochemistry
Doi: https://doi.org/10.1111/jnc.13514
     
 

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