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Flag tag polyclonal antibody
货号 | 规格 | 价格(元) | 货期 | ||
GAB800501 | 100ul | ¥600.00 | 2~3天 | ||
GAB800502 | 200ul | ¥900.00 | 2~3天 | ||
GAB800503 | 1000ul | 询价 | 2~3天 | ||
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Background | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. | ||||
Description | The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag | ||||
Source | This antibody is produced by immunizing rabbits with a synthetic peptide DYKDDDDK (KLH-coupled). | ||||
Applications | WB 1:1000-2000, ICH 1:500-2000. Optimal dilution has to be determined by the user. | ||||
Applicable Species | Mouse, rat, human. | ||||
Specifications | Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography. | ||||
Storage conditions | Store at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃). |